Founder and CEO: +31 (0)6 422 66633 In addition to macrophage ablation, osteoclast ablation occurred. In this study, the well-established C57BL/KaLwRijHsd (KaLwRij) murine model of myeloma was utilized in combination with clo-lip–mediated macrophage depletion to assess the role of mature macrophages in the initial establishment of MM PC within the BM. This molecule has a very short half life when released in the circulation, but does not easily … Clodronate liposomes (100 μL per 20 g body weight) or PBS liposomes were administered by i.p. Encapsome® is formulated and prepared identically to Clodrosome® except that clodronate is not added to the liposomes. At LIPOSOMA research liposomes we manufacture and provide liposomal formulations for scientific purposes, specifically clodronate liposomes, control (empty) liposomes and fluorescent liposomes. Use this unique QR (Quick Response) code with your smart device. I ordered liposomes (both PBS controls and clodronate) from Formumax (liposomeexperts.com) and inject 0.1mg/g of b.w. Encapsula NanoSciences only provides products, services or other information for use in research laboratories by, or under the technical supervision of, research personnel experienced in their safe handling and application. How to handle Clodronate liposomes and control liposomes: (#20) How to handle the Clodronate liposomes upon arrival The suspension of Clodronate liposomes as well as that of control liposomes should be used as they are, without dilution or any other treatment. Clodronate (dichloromethylene bisphosphonate) and gallium in liposomes are macrophage suppressive agents, and thus possibly useful in inflammatory diseases. While liposomal clodronate usually demonstrates an IC 50 (concentration required to kill 50% of the cells or limit the growth of cells by 50%) of an order of magnitude higher than free clodronate, the experimental protocol should take into account the possibility of clodronate release from liposomes or dead/dying cells reaching a concentration that will effect the cultured cells. In order to decipher the role of macrophages in vivo, it could be necessary to establish a model of macrophage depletion in the whole animal. Clodronate (Systemic) VA CLASSIFICATION Primary: HS302 Commonly used brand name(s): Bonefos. In recent times gradually more time had to be spent to financial in stead of scientific matters. These terms apply to any and all institutions or companies ordering from Encapsula, regardless of the internal payment policies of the purchasing organization. In this study, we aim to determine whether peritoneal injection of clodronate liposomes has any beneficial effect on systemic glucose homeostasis/insulin sensitivity and whether macrophage content in visceral adipose tissue will be reduced in diet-induced obese (DIO) mice. Micron sized liposomes engineered specifically to target macrophages in different tissues and organs. Systemically administered clodronate encapsulated in large multilamellar vesicles (MLV), sterically stabilized with polyethylene glycol MS400 stearate (PEG-S), is capable of suppressing paw inflammation in rat AA [ 23 ]. We reserve the right to validate the source of inquiries or orders prior to any action on our part. Clodronate molecules are encapsulated in small MLV liposomes for superior activity, physical and chemical stability, and convenience of use and handling. The method is specific with respect to phagocytic cells of the mononuclear phagocyte system (MPS) for the following reasons: (1) The natural fate of liposomes is phagocytosis. This law also applies to those ordering from outside the United States. BV = Blood vessels (circulation) BR = Brain. Clodronate liposomes can ablate macrophages by inducing apoptosis. extensions. FormuMax offers preformed liposome reagents to R&D organizations involved in drug delivery using liposomes. Clodronate encapsulated in liposomes is also toxic to macrophages in vivo and may therefore be of use in the treatment of inflammatory diseases. Clodronate Liposomes & Control Liposomes (PBS) Fluorescent DiI liposomes; Clodronate Liposomes & Fluorescent DiI liposomes; Information. al. ... PURCHASE ORDERS, FDA, IMPORT PERMITS, INVOICES. E-mails will be answered within 2 hours of receipt. Suite 360 Both types of Clophsome ® are offered in the standard suspension form fo rthe convenience of use, the high potency form fo rdosing of high cotent with limited small volume and the lyophilized forms for the long shelf-life. Lyophosome™-PEGylated catalog products contain anionic, cationic and neutral freeze-dried liposomes containing 5 mol percent DSPE-PEG(2000) lipid without any encapsulated molecules which are suitable for many different types of experiments. Our team of liposome experts and support staff has successfully served thousands of … Liposomes are artificially prepared lipid vesicles, consisting of concentric phospholipid bilayers entrapping aqueous compartments. Office Manager: [email protected] COLLABORATION. GU = Gut (intestines) They can be stored at 4 degrees Celcius when not used within a few days upon arrival. If Encapsula does not receive payment in full within 30 days of the invoice date, subsequent orders may not be accepted until the account is reconciled. Clodronate reduced circulating monocytes to 42.3% of control (liposomes without clodronate) 24h after injection. To better serve our international clients, we take calls 24 hours a day — 7 days a week. If you have any questions about placing an order with Encapsula NanoSciences, please Contact Us. All pertinent company information is in the CCR database. every subsequent injection. Each standard macrophage depletion kit contains one vial of Clodronate encapsulated liposomes (Clodrosome) and one vial of plain liposome for control (Encapsome) with the same lipid composition as Clodrosome. formulations sold on the website are for research purposes only. Encapsula NanoSciences is also registered with the ARIBA supplier network. Never use these products in humans, companion animals or any animals other than those maintained in a licensed research facility. Flow cytometry after laser-CNV induction revealed a smaller F4/80+CD11b+ macrophage population in the RPE/choroid complex of Hrh4-/- mice than in wild-type mice. The United States Food and Drug Administration (FDA) dictates that Encapsula NanoSciences only sell regulated products and/or provide services involving regulated products to those affiliated with universities, research institutes or biotechnology/pharmaceutical companies actively engaged in research with a licensed animal facility. Clodronate liposomes, saline liposomes, or saline alone was injected under the ovarian bursa in gonadotropin-primed adult mice, either 84 h (Day −3) or 36 h (Day −1) before ovulation. They can be used to encapsulate strongly hydropihilic molecules solved in aqueous solutions, such as clodronate, a non-toxic bisphosphonate, developed for … Interestingly, depletion of macrophages and osteoclasts via clodronate treatment had differential effects based on skeletal location. Macrophage Depletion Reagents (Clodrosome®), Full Fluorescent Macrophage Depletion Kits, Mannosylated Fluorescent Macrophage Depletion Kits, Mannosylated Full Fluorescent Macrophage Depletion Kits, Liposomal Doxorubicin (Doxosome™ and Immunodox®), Liposomes for DNA/RNA Delivery (Genesome®), Lyophilized Liposomes for Formulators (Lyophosome™), Standard Macrophage Depletion Kit (Clodrosome® + Encapsome®), Full Macrophage Depletion Kit (w/Fluoroliposome®-DiA), Full Macrophage Depletion Kit (w/Fluoroliposome®-DiD), Full Macrophage Depletion Kit (w/Fluoroliposome®-DiI), Full Macrophage Depletion Kit (w/Fluoroliposome®-DiO), Full Macrophage Depletion Kit (w/Fluoroliposome®-DiR), Mannosylated Macrophage Depletion Kit (m-Clodrosome® + m-Encapsome®), Three-Color Mannosylated Fluoroliposome® Kit, Five-Color Mannosylated Fluoroliposome® Kit, Mannosylated Fluorescent-DiA Macrophage Depletion Kit, Mannosylated Fluorescent-DiD Macrophage Depletion Kit, Mannosylated Fluorescent-DiI Macrophage Depletion Kit, Mannosylated Fluorescent-DiO Macrophage Depletion Kit, Mannosylated Fluorescent-DiR Macrophage Depletion Kit, Mannosylated Full Macrophage Depletion Kit (w/Fluoroliposome®-DiA), Mannosylated Full Macrophage Depletion Kit (w/Fluoroliposome®-DiD), Mannosylated Full Macrophage Depletion Kit (w/Fluoroliposome®-DiI), Mannosylated Full Macrophage Depletion Kit (w/Fluoroliposome®-DiO), Mannosylated Full Macrophage Depletion Kit (w/Fluoroliposome®-DiR), Sulfhydryl Reactive Doxorubicin Loposomes. ... PURCHASE ORDERS, FDA, IMPORT PERMITS, INVOICES. After fusion with lysosomes (L) containing phospholipases (arrowheads), the latter disrupt the bilayers of the liposomes. Submit a purchase order by FAX to 615-250-8747, or e-mail a PDF of the purchase order to info@encapsula.com with “Clodrosome Order” in the subject line. Any use of these products in humans Ovulation rates were determined by counting the number of oocytes released. Clodronate is clinically used for the treatment of osteolytic bone diseases. A liposome mediated macrophage "suicide" approach has been developed, based on the liposome mediated internalization of the small hydrophilic molecule clodronate in macrophages J Leukoc Biol 62:702, 1997. Office Manager: [email protected] COLLABORATION. The clodronate-loaded liposomes and PBS-containing liposomes are ready for use, but the solutions that usually kept in 4 °C need to be warmed up to room temperature before injections. ... spleen, liver, lungs, brain, gut, peritoneal cavity, lymph nodes/vessels) and blood is the use of a clodronate-liposome solution.Here, we describe the protocol used to deplete efficiently macrophages in mouse bone marrow. Each standard macrophage depletion kit contains one vial of Clodronate encapsulated liposomes (Clodrosome) and one vial of plain liposome for control (Encapsome) with the same lipid composition as Clodrosome. Encapsome® control liposomes are recognized and phagocytosed by the same mechanism as Clodrosome®. Clodronate Liposomes Abstracts ... mice that had intraperitoneally received clodronate liposome. This will simplify your paperwork. Clodronate liposomes decreased tumor number by ∼36% and specifically large (≥1 mm) tumors by ∼36% ( P < 0.05). Liposomes encapsulating the bisphosphonate clodronate can be used for the transient suppression of macrophage functions. Unencapsulated clodronate cannot cross the cell membrane to initiate cell death. Furthermore, as expected, clodronate was internalized by J774 macrophages more efficiently when encapsulated in liposomes, since the size of the ultraviolet absorbance peak owing to the metabolite was similar in extracts from cells treated for 24 h with 15 μM liposome‐encapsulated clodronate and in extracts from cells treated for 48 h with 250 μM free clodronate. Encapsula NanoSciences LLC Their roles may include the phagocytosis and degradation of foreign organisms, tissue remodeling, and the regulation of local immune and inflammatory responses. This was consistent with a decrease in gene expression of EMR1 in the colon tissue and polyp tissue as well as expression of select markers associated with M1 (IL-6) and M2 macrophages (IL-13, IL-10, TGFβ, CCL17) in the colon tissue ( P < 0.05). educational purposes. In order to decipher the role of macrophages in vivo, it could be necessary to establish a model of macrophage depletion in the whole animal. While liposomal clodronate usually demonstrates an IC 50 (concentration required to kill 50% of the cells or limit the growth of cells by 50%) of an order of magnitude higher than free clodronate, the experimental protocol should take into account the possibility of clodronate release from liposomes or dead/dying cells reaching a concentration that will effect the cultured cells. However, the majority of the papers investigate the effect of clodronate liposomes on rat, mouse and sheep models of osteo- and rheumatoid arthritis. Weight: 02 kg: Quantity: 5 ml (5ml Clodronate Liposomes), 10 ml (10ml Clodronate Liposomes), 15 ml (15ml Clodronate Liposomes), 20ml (20ml Clodronate Liposomes), 25 ml (25ml Clodronate Liposomes), 30 ml (30ml Clodronate Liposomes), 40 ml (40ml Clodronate Liposomes), 50 ml (50ml Clodronate Liposomes), 60 ml (60ml Clodronate Liposomes), 70 ml (70ml Clodronate Liposomes), 80 ml (80ml Clodronate … Stichting Clodronate Liposomes staat bij de Kamer van Koophandel geregistreerd met het KVK nummer 34173596. Read More. A liposome mediated macrophage "suicide" approach has been developed, based on the liposome mediated internalization of the small hydrophilic molecule clodronate in macrophages J Leukoc Biol 62:702, 1997. Any commercial use of the content of the website is legally prohibited We have developed a macrophage 'suicide' technique, using the liposome mediated intracellular delivery of dichloromethylene-bisphosphonate (Cl2MBP or clodronate). Clodronate Liposomes & Control Liposomes (PBS) Fluorescent DiI liposomes; Clodronate Liposomes & Fluorescent DiI liposomes; Information. Unlike free clodronate, clodronate-liposomes (clo-lip) globally deplete macrophages and other phagocytic cells , . Online in our webshop. We offer one year shelf-life guarantee from the time of delivery to the date of reconstitution. Clophosome ®-A - Clodronate Liposomes (Anionic) . This process may be overturned if clodronate liposomes are injected intravenously. 9 The average size of liposomes … Macrophage Depletion Kit (Mannosylated) Clodrosome®, Encapsome®, Doxosome®, Immunosome®, Genesome®, Fluoroliposome®, ATPsome®, Cellsome®, Immunodox® and Immunofluor® De informatie die je hier vindt over Stichting Clodronate Liposomes komt deels vanuit Graydon en deels vanuit de KvK. or animals/ pets for treatment purposes is legally prohibited by U S Food and Drug Administration. Clodronate molecules are encapsulated in small MLV liposomes for superior activity, physical and chemical stability, and convenience of use. Second, in our experience 6 like in that of others, 7, 8 when clodronate liposomes are injected i.p., they affect not only liver but also intra‐abdominal adipose tissue macrophages (ATMs) such as epididymal and omental ATMs. The kits come in 2 different varieties: standard kits and mannosylated kits (mainly to target central nervous system). The degree of depletion depends on the route of injection and the amount of injected clodronate liposomes. Depleting Macrophages In Vivo with Clodronate-Liposomes. It is generally believed that bisphosphonates are not metabolized. Encapsome® is formulated and prepared identically to Clodrosome® except that clodronate is not added to the liposomes. * Not commercially available in the U.S. Category: Bone resorption inhibitor— Liposomes, encapsulating the Clodronate molecules (squares), are ingested by macrophages via endocytosis. Please include in the order an email address, specifications, a shipping address, a billing address, email address from Account Payable and the Purchase Order number. to induce systemic macrophage depletion in C57BL/6 mice [35]. Liposomes. If you are ordering from government research laboratories, such as National Institutes of Health (NIH), please keep in mind that Encapsula NanoSciences is registered with the CCR. * Clodronate molecules are encapsulated in small MLV liposomes for superior activity, physical and chemical stability, and convenience of use. Phagocytosed by the same mechanism as Clodrosome® e-mail account, did not work as.. 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The only proven effective method for specifically targeting clodronate to phagocytes in vivo and may therefore be of use the! Counting the number of oocytes released with lysosomes ( L ) containing phospholipases ( arrowheads ), content!, depletion of macrophages in the CCR database and inject 0.1mg/g of b.w in C57BL/6J mice and oral... One year shelf-life guarantee from the time of the website can only be used for the selective of... Clodronate is not added clodronate liposomes purchase the liposomes about placing an order with encapsula NanoSciences accepts verified PURCHASE orders,,...